Monoclonal Anti-Nicotinic Acetylcholine Receptor (α₇ Subunit) antibody produced in mouse

Nicotinic acetylcholine receptors (nAChRs) function as ligand-gated channels that modulate endoplasmic reticulum stress and neurotransmitter release. These receptors also control synaptic signaling in ganglia and muscles. The α7 form of nAChR is expressed in mitochondria and regulates Ca²⁺ transport and the release of cytochrome c. When expressed in non-excitable cells, α7 nAChR mediates cell growth and survival
Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is specific for native and denatured α7 subunits in chick, as well as for the denatured α7 subunits in rats and humans. The antibody does not bind well to the native form of the mammalian α7 subunit. The product can be used in chick, rat and human tissues.

native and denatured α₇ subunit (amino acids 380-400, binds α-bungarotoxin) of the nicotinic acetylcholine receptor from chicken and the denatured α₇ subunit from rat.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is suitable for use in immunocytochemistry (at 1:3000-1:50 dilutions using paraformaldehyde fixed, cultured rat chromaffin cells) , and immunohistochemistry (at 1:3,000 dilutions using frozen sections).

Solution in 20 mM Na₃PO₄, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.

Sold with permission of the Salk Institute

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