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M2378

Sigma-Aldrich

Mucin from porcine stomach

Type II

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Synonym(s):
MUC
CAS Number:
84082-64-4
EC Number:
282-010-7
MDL number:
MFCD00131629
NACRES:
NA.61

biological source

Porcine stomach

Quality Level

200

type

Type II

form

powder

composition

bound sialic acid, ≤1.2%

technique(s)

cell culture | mammalian: suitable

solubility

1 M NaOH: soluble 20 mg/mL

UniProt accession no.

A0A5G2QSD1

storage temp.

2-8°C

Gene Information

pig ... MUC2(100127145)

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General description

Mucin is majorly composed of carbohydrate units, crucial for structural architecture and conformation. The monomer of mucin is 640 kDa, with protein contributing to only 20% of the molecular weight. The carbohydrate component is attached to the threonine and serine side chains. Mucins are classified into secreted and transmembrane mucins.
Mucins or mucus glycoproteins are the main macromolecular components of mammalian mucus. It was also used in a study to evaluate the sputum smears concentrated by cyto-centrifugation for detection of acid-fast bacilli.

Application

Mucin from porcine stomach has been used:
  • in the standard curve generation for quantifying mucin-2 generated during mucosal inflammation in mice
  • as a component of digestion fluid solutions in in vitro human digestion model
  • as a component of simulated saliva fluid

Mucin from porcine stomach was used in studies on the binding site of the galactose-specific agglutinin PA-IL from Pseudomonas aeruginosa.

Biochem/physiol Actions

Mucin has wide pharmacological applications in drug delivery. Mucins play a protective role in inflammation. Deregulation in mucin synthesis contributes to tumorigenesis and chronic inflammation.
Mucus forms the protective cover for all epithelial surfaces. The major structural component of mucus is gel-forming mucins. The mucus layer covering the intestinal epithelium has the MUC2 mucin as its central molecule. MUC2 is produced by goblet cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What does the "Type" designation in Mucin from porcine stomach mean?

    The type designation does not have any particular biological significance.  It is used to emphasize differences in the listings of this product.

  6. What are the differences between Mucin from porcine stomach Products M2378 and M1778?

    Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website.

  7. Is Product M2378, Mucin from porcine stomach, a purified preparation of mucin?

    No. This product is a crude preparation of mucin; it is a mixture of glycoproteins.  It is obtained by digestion of hog stomach with pepsin, followed by precipitation and other steps.

  8. How can I solubilize Product M2378, Mucin from porcine stomach?

    This crude mucin may not be readily soluble in solution. At 20 mg/mL in 1 M sodium hydroxide, turbid suspensions are observed. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.

  9. What is the molecular weight of Product M2378, Mucin from porcine stomach?

    This product is a crude mixture of glycoproteins. The sugar and amino acid composition of the mucin has not been determined.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

An optimized purification process for porcine gastric mucin with preservation of its native functional properties
Schomig VJ, et al.
Royal Society of Chemistry Advances, 6(50), 44932-44943 (2016)
Melinda A Engevik et al.
ACS infectious diseases, 7(5), 1126-1142 (2020-11-13)
It is widely accepted that the pathogen Clostridioides difficile exploits an intestinal environment with an altered microbiota, but the details of these microbe-microbe interactions are unclear. Adherence and colonization of mucus has been demonstrated for several enteric pathogens and it
Structural investigation of porcine stomach mucin by X-ray fiber diffraction and homology modeling
Veluraja K, et al.
Biochemical and biophysical research communications, 406(4), 570-573 (2011)
Maria Schei Haugan et al.
Scientific reports, 8(1), 14961-14961 (2018-10-10)
The efficacy of most antibiotics is dependent on active bacterial growth, yet little is known about the growth dynamics during infection. Therefore, means to measure in-host bacterial growth rate is of importance. Here, we use chromosome replication as readout for
Genetic mouse models with intestinal-specific tight junction deletion resemble an ulcerative colitis phenotype
Stremmel W, et al.
Journal of Crohn's & Colitis, 11(10), 1247-1257 (2017)
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