M2634
Malic Dehydrogenase from porcine heart
buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
Synonym(s):
L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Specific activity:
600-1000 units/mg protein (biuret)
form
buffered aqueous glycerol solution
Quality Segment
specific activity
600-1000 units/mg protein (biuret)
cation traces
NH4+: ≤10 μg/mg protein
foreign activity
Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
shipped in
wet ice
storage temp.
2-8°C
General description
Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.
Mitochondrial Isozyme
Application
Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.
Biochem/physiol Actions
Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.
Physical form
Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5
Other Notes
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
Still not finding the right product?
Explore all of our products under Malic Dehydrogenase from porcine heart
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.