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Merck
CN

M3633

4-Methylumbelliferyl β-D-glucopyranoside

β-glucosidase substrate, fluorogenic, ≥99% (HPLC), powder

Synonym(s):

MUD

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About This Item

Empirical Formula (Hill Notation):
C16H18O8
CAS Number:
Molecular Weight:
338.31
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
242-736-7
MDL number:
Beilstein/REAXYS Number:
94672
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Product Name

4-Methylumbelliferyl β-D-glucopyranoside, β-glucosidase substrate

InChI key

YUDPTGPSBJVHCN-YMILTQATSA-N

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)ccc12

assay

≥99% (HPLC)

form

powder

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 316 nm; λem 372 nm (pH 9.1)
λex 360 nm; λem 449 nm (Reaction product)

storage temp.

−20°C

Quality Level

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Application

4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:
  • in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
  • in β-glucosidase assay during yeast fermentation
  • to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Determinants on an efficient cellulase recycling process for the production of bioethanol from recycled paper sludge under high solid loadings
Gomes D, et al.
Biotechnology for Biofuels, 11(1), 111-111 (2018)
Florent Malard et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 21(6), 1059-1067 (2015-02-25)
This retrospective report compared the 4-year outcomes of allogeneic stem cell transplantation (allo-SCT) in 651 adult patients with acute myeloid leukemia receiving a reduced-intensity (RIC) or nonmyeloablative conditioning (NMA) regimen according to the type of unrelated donors. These were either
Assessing microbial safety of drinking water improving approaches and methods: Improving approaches and methods, 253-253 (2003)
Samantha G Scharenberg et al.
Nature communications, 11(1), 3327-3327 (2020-07-06)
Gaucher disease is a lysosomal storage disorder caused by insufficient glucocerebrosidase activity. Its hallmark manifestations are attributed to infiltration and inflammation by macrophages. Current therapies for Gaucher disease include life-long intravenous administration of recombinant glucocerebrosidase and orally-available glucosylceramide synthase inhibitors.
HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
Tol MJ, et al.
Autophagy, 14(3), 437-449 (2018)

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