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M4530

Sigma-Aldrich

Medium 199

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
M199 Medium, TCM 199
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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General description

Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • as a suspension media to maintain the primary neuronal cultures
  • as a component of the collagen gel for ex vivo embryonic heart culture
  • to maintain the ovarian tissue sections as part of the slow-freezing protocol

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

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Li Ma et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 72(4), 489-498 (2016-08-28)
We previously reported that pretreatment with the potent antioxidant TEMPOL improves mitochondrial function and restores preconditioning in the aging heart. Because mitophagy is implicated in cardiac preconditioning and declines with age, this study was designed to investigate how age influences
Patrick C Baer et al.
International journal of molecular sciences, 19(6) (2018-06-13)
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens
Amanda Fanous et al.
Plastic and reconstructive surgery, 143(3), 760-767 (2019-01-03)
Keloid scarring is a serious condition that mostly affects patients of African or Asian descent. Often disfiguring, this condition can have devastating psychosocial consequences. To date, no treatment modality has been proven ideal. The authors' objectives were (1) to determine
Immacolata Castellano et al.
Oxidative medicine and cellular longevity, 2018, 2087373-2087373 (2018-06-01)
Chronic hyperglycemia is associated with oxidative stress and vascular inflammation, both leading to endothelial dysfunction and cardiovascular disease that can be weakened by antioxidant/anti-inflammatory molecules in both healthy and diabetic subjects. Among natural molecules, ovothiol A, produced in sea urchin
Vladimir Jovasevic et al.
Journal of virology, 82(7), 3311-3319 (2008-01-25)
In this report we propose a model in which after the herpes simplex virus (HSV) capsid docks at the nuclear pore, the tegument protein attached to the capsid must be cleaved by a serine or a cysteine protease in order
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Articles

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

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Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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