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M5650

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
EMEM, MEM
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used to culture:
  • HeLa cells
  • Madin-Darby canine kidney (MDCK) cells
  • chondrosarcoma cell line

This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Regulatory Information

动植物来源培养基

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Christopher G Mills et al.
Scientific reports, 7(1), 14824-14824 (2017-11-03)
We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in
3D cell sheets formed via cell-driven buckling-delamination of patterned thin films
Oyama T, et al.
Materials & Design, 208, 109975-109975 (2021)
Bryan Tay et al.
PloS one, 14(3), e0213751-e0213751 (2019-03-12)
Voltage-gated sodium channels (NaVs) are key therapeutic targets for pain, epilepsy and cardiac arrhythmias. Here we describe the development of a no-wash fluorescent sodium influx assay suitable for high-throughput screening and characterization of novel drug leads. Addition of red-violet food
Junjie Jing et al.
International journal of clinical and experimental medicine, 8(8), 13127-13135 (2015-11-10)
Tectonic family member 1 (TCTN1) encodes a member of the tectonic family which are evolutionarily conserved secreted and transmembrane proteins, involving in a diverse variety of developmental processes. It has been demonstrated that tectonics expressed in regions that participate in
Emanuela Profilo et al.
Biochimica et biophysica acta, 1863(6), 1422-1435 (2017-02-27)
The mitochondrial aspartate-glutamate carrier isoform 1 (AGC1) catalyzes a Ca
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MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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