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M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Synonym(s):

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide

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10000 EA
CN¥2,255.11

CN¥2,255.11


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10000 EA
CN¥2,255.11

About This Item

Empirical Formula (Hill Notation):
C18H16BrN5S
CAS Number:
Molecular Weight:
414.32
Beilstein:
4081397
EC Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

CN¥2,255.11


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product line

BioReagent

Quality Level

Assay

≥97.5% (HPLC)

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

mp

195 °C (dec.) (lit.)

solubility

H2O: 5 mg/mL

storage temp.

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

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This Item
CLS4844CLS4846CLS4866
feature

DNase free, barrier: no, endotoxin free, Bulk Pack, RNase free, filter: no, disposable

feature

DNase free, RNase free, barrier: no, disposable, endotoxin free, filter: no, Bulk Pack

feature

DNase free, RNase free, barrier: no, disposable, endotoxin free, filter: no, Bulk Pack

feature

DNase free, RNase free, barrier: no, disposable, endotoxin free, filter: no, graduation, Bulk Pack

manufacturer/tradename

Corning 4845

manufacturer/tradename

Corning 4844

manufacturer/tradename

Corning 4846

manufacturer/tradename

Corning 4866

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

polypropylene , yellow tip

material

natural tip, polypropylene

material

blue tip, polypropylene

material

polypropylene , yellow tip

packaging

bag of 1000

packaging

bag of 1000, case of 10000

packaging

bag of 1000, case of 10000

packaging

bag of 1000, case of 1000

volume range

1-200 μL

volume range

1-200 μL

volume range

1-1000 μL

volume range

1-200 μL

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay).[1][2][3][4] It has also been used to determine cell proliferation.[5]

Pictograms

Health hazardExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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