M5691
Anti-MAGI-1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat
technique(s)
microarray: suitable
western blot: 1:500 using rat brain extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAGI1(9223)
mouse ... Magi1(14924)
rat ... Cnksr3(308113)
General description
The MAGUK (Membrane Associated Guanylate kinase) family of proteins localize to regions of cell-cell contact, such as tight junctions in epithelial cells and synaptic junctions in neurons. In epithelia, membrane associated guanylate kinase, WW And PDZ domain containing 1 (MAGI-1) is localized at tight junctions and is expressed in three isoforms (MAGI-1a, MAGI-1b, and MAGI-1c). MAGI-1 was first identified in mouse as a protein interacting with K-RasB (Ras-like protein rasB).
Immunogen
synthetic peptide corresponding to amino acids 282-296 of MAGI-1 with a carboxy terminal added cysteine residue conjugated to maleimide-activated KLH. The sequence is conserved in human and mouse.
Application
Anti-MAGI-1 antibody produced in rabbit has been used in
- immunoblotting
- western blotting
- immunostaining
Biochem/physiol Actions
Membrane associated guanylate kinase, WW And PDZ domain containing 1 (MAGI-1) interacts with β-catenin. Neuroepithelial cell-transforming gene 1(nNET1) and actin binding proteins act as binding partners of MAGI-1. Interestingly, MAGI-1c is present in nucleus, suggesting that MAGI-1 may participate in the transmission of regulatory signals from the cell surface to the nucleus.
Recognizes rat MAGI-1 by immunoblotting (approx. 170, 130, and 120 kDa) and does not cross react with MAGI-2 or MAGI-3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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MAGI-1, a candidate stereociliary scaffolding protein, associates with the tip-link component cadherin 23
Xu Z, et al.
The Journal of Neuroscience, 28(44), 11269-11276 (2008)
MAGI-1: A Widely Expressed, Alternatively Spliced Tight Junction Protein
Dobrosotskaya IY, et al.
Biochemical and Biophysical Research Communications, 270(3), 903-909 (2000)
Shuqin Jia et al.
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 29(1), 25-35 (2017-04-05)
To explore the association of membrane-associated guanylate kinase inverted 1 (MAGI1) with gastric cancer (GC) and the related molecular mechanisms. The reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were utilized to measure the MAGI1 expression level in GC tissues.
MAGI-1: A Widely Expressed, Alternatively Spliced Tight Junction Protein
Laura RP, et al.
Experimental Cell Research, 275(2), 155-170 (2002)
Jun-Ichi Abe et al.
JCI insight, 4(7) (2019-04-05)
The possible association between the membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1) and inflammation has been suggested, but the molecular mechanisms underlying this link, especially during atherogenesis, remain unclear. In endothelial cells (ECs) exposed to disturbed flow (d-flow), p90
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