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M5814

MKK6/SKK3, active human

Name: MKK6/SKK3, active human
Brand: SIGMA

≥80% (SDS-PAGE), recombinant, expressed in E. coli, Maltose binding protein tagged, buffered aqueous solution

Synonym(s):

MAP Kinase Kinase 6, MAP Kinase Kinase 6/SKK 3

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.32

MDL number:

MFCD03457508

Assay:

≥80% (SDS-PAGE)

Recombinant:

expressed in E. coli

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recombinant

expressed in E. coli

Quality Level

200

assay

≥80% (SDS-PAGE)

form

buffered aqueous solution

mol wt

81 kDa

UniProt accession no.

P52564

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MAP2K6(5608)

Application

Active enzyme for study of stress-activated protein kinases.

May be used for activation of p38α followed by phosphorylation of MBP.

Biochem/physiol Actions

Active in the phosphorylation of p38/SAPK2a

Physical form

Solution in approx. 1 mg/ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.15 M NaCl, 0.03% Brij^® L23, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine.

Other Notes

1 unit will activate 1 unit of p38/SAPK2 per minute. 1 unit of active p38/SAPK2 will incorporate 1 nanomole of phosphorus into myelin basic protein per minute.

Legal Information

Brij is a registered trademark of Croda International PLC

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Activation of stress-activated protein kinase-3 (SAPK3) by cytokines and cellular stresses is mediated via SAPKK3 (MKK6); comparison of the specificities of SAPK3 and SAPK2 (RK/p38).

A Cuenda et al.

The EMBO journal, 16(2), 295-305 (1997-01-15)

Stress-activated protein kinase-3 (SAPK3), a recently described MAP kinase family member with a wide-spread tissue distribution, was transfected into several mammalian cell lines and shown to be activated in response to cellular stresses, interleukin-1 (IL-1) and tumour necrosis factor (TNF)

Global Trade Item Number

SKU	GTIN
M5814-5UG	04061837906510

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