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M5904

Sigma-Aldrich

Mineral oil

BioReagent, for molecular biology, light oil

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Synonym(s):
light mineral oil
CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

light oil

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
  • as a continuous phase to prevent droplet coalescence in emulsification system

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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A quantitative analysis of contractility in active cytoskeletal protein networks
Bendix PM, et al.
Biophysical Journal, 94(8), 3126-3136 (2008)
Microfluidic flow-focusing in ac electric fields
Tan SH, et al.
Lab on a chip, 14(6), 1099-1106 (2014)
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated
Process Fluids for Abrasive Machining
Tribology of Abrasive Machining Processes, 531-585 (2004)
Paraffin wax as a vapor barrier for the PCR.
Sparkman DR
Genome Research, 2(2), 180-181 (1992)
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