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M6190

Monoclonal Anti-MYOD1 antibody produced in mouse

clone 5.2F, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Myogenic Differentiation Antigen 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01323984
Conjugate:
unconjugated
Clone:
5.2F, monoclonal
Application:
ICC, IHC (f), IHC (p), IP, WB
Citations:
8
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.2F, monoclonal

form

buffered aqueous solution

mol wt

antigen 34 kDa

species reactivity

human, rat, chicken, mouse

concentration

1.0 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL, immunohistochemistry (frozen sections): 2-4 μg/mL, immunoprecipitation (IP): 2 μg using 1 mg protein lysate, western blot: 1 μg/mL (reacts with the ~45 kDa protein)

isotype

IgG2a

UniProt accession no.

P15172

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYOD1(4654)
mouse ... Myod1(17927)
rat ... Myod1(337868)

General description

Myogenic differentiation antigen 1 (MYOD1) is a nuclear protein which is expressed in skeletal muscles. It is part of the basic helix-loop-helix (bHLH) family of transcription factors. The gene encoding it is localized on human chromosome 11.

Immunogen

recombinant mouse MyoD1 protein.

Application

Monoclonal Anti-MYOD1 antibody produced in mouse has been used in:
  • immunofluorescence staining at a 1:50 dilution
  • western blotting
  • immunostaining at a 1:300 dilution

Biochem/physiol Actions

Myogenic differentiation antigen 1 (MYOD1) maybe involved in recruitment of enzymes like acetyltransferases and methyltransferases to myogenic enhancers in the human genome. It takes part in the regeneration of muscles and mediates muscle cell differentiation by activating cell cycle arrest.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
低风险生物材料

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000501209
0000501209
SLCD7429
SLCD7428
SLCD6649

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Related Content

Data Sheet - M6190

Instructions

Xiangyu Sui et al.
Frontiers in oncology, 12, 1040112-1040112 (2022-11-18)
Skeletal muscle atrophy is the major hallmark of cancer cachexia. The mechanisms underlying muscle wasting remain elusive in cachectic patients. Our research seeks to identify differentially expressed genes (DEGs) between non-cachectic and cachectic cancer patients and elucidate their functions. We
Shujie Chen et al.
Bio-protocol, 9(14), e3313-e3313 (2019-07-20)
Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of
Bahar Shahidi et al.
JOR spine, 3(2), e1087-e1087 (2020-07-03)
Many chronic musculoskeletal conditions are associated with loss of muscle volume and quality, resulting in functional decline. While atrophy has long been implicated as the mechanism of muscle loss in these conditions, recent evidence has emerged demonstrating a degenerative phenotype
View All Related Papers

Global Trade Item Number

SKUGTIN
M6190-100UG04061834060741