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M6190

Monoclonal Anti-MYOD1 antibody produced in mouse

Name: Monoclonal Anti-MYOD1 antibody produced in mouse
Brand: SIGMA

clone 5.2F, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Myogenic Differentiation Antigen 1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01323984

Conjugate:

unconjugated

Clone:

5.2F, monoclonal

Application:

ICC, IHC (f), IHC (p), IP, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.2F, monoclonal

form

buffered aqueous solution

mol wt

antigen 34 kDa

species reactivity

human, rat, chicken, mouse

concentration

1.0 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL, immunohistochemistry (frozen sections): 2-4 μg/mL, immunoprecipitation (IP): 2 μg using 1 mg protein lysate, western blot: 1 μg/mL (reacts with the ~45 kDa protein)

isotype

IgG2a

UniProt accession no.

P15172

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYOD1(4654)
mouse ... Myod1(17927)
rat ... Myod1(337868)

Description

General description

Myogenic differentiation antigen 1 (MYOD1) is a nuclear protein which is expressed in skeletal muscles. It is part of the basic helix-loop-helix (bHLH) family of transcription factors. The gene encoding it is localized on human chromosome 11.

Immunogen

recombinant mouse MyoD1 protein.

Application

Monoclonal Anti-MYOD1 antibody produced in mouse has been used in:

immunofluorescence staining at a 1:50 dilution
western blotting
immunostaining at a 1:300 dilution

Biochem/physiol Actions

Myogenic differentiation antigen 1 (MYOD1) maybe involved in recruitment of enzymes like acetyltransferases and methyltransferases to myogenic enhancers in the human genome. It takes part in the regeneration of muscles and mediates muscle cell differentiation by activating cell cycle arrest.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c