M6320
Monoclonal Anti-MAGE-1 antibody produced in mouse
~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Melanoma-associatiated antigen 1
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About This Item
Conjugate:
unconjugated
Clone:
MA454, monoclonal
Application:
ELISA (i), IHC, WB
Citations:
9
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MA454, monoclonal
form
buffered aqueous solution
mol wt
antigen ~46 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 5-10 μg/mL using total cell extract of SKML37 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAGEA1(4100)
General description
Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen. MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28. Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.
Immunogen
recombinant human MAGE-1.
Application
Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:
- ELISA
- immunoblotting
- immunohistochemistry
Biochem/physiol Actions
The antibody does not recognize MAGE-2 and MAGE-3.
The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC). Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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Clinical Significance of Melanoma Antigen-Encoding Gene-1 (MAGE-1) Expression and its Correlation with Poor Prognosis in Differentiated Advanced Gastric Cancer
Ogata MDK, et al.
Annals of Surgical Oncology (2011)
The MAGE Proteins: Emerging Roles in Cell
Cycle Progression, Apoptosis, and
Neurogenetic Disease
Cycle Progression, Apoptosis, and
Neurogenetic Disease
Barker PA and Salehi A
Journal of Neuroscience Research (2002)
Madhav V Dhodapkar et al.
Cancer immunity, 3, 9-9 (2003-07-24)
Cancer/testis (CT) antigens are expressed in several malignant tumors, but not in normal tissues except for testicular germ cells. The expression of CT antigenic proteins in malignant gammopathies has not been characterized. We examined the expression of a panel of
Jiang Xiao et al.
World journal of gastroenterology, 10(13), 1849-1853 (2004-06-29)
To date, dozens of melanoma-associated antigens (MAGEs) have been identified and classified into 2 subgroups, I and II. Subgroup I consists of antigens which expression is generally restricted to tumor or germ cells, also named as cancer/testis (CT) antigen. Proteins
Oliver Goodyear et al.
Blood, 116(11), 1908-1918 (2010-06-10)
Epigenetic therapies, including DNA methyltransferase and histone deacetylase inhibitors, represent important new treatment modalities in hematologic malignancies, but their mechanism of action remains unknown. We reasoned that up-regulation of epigenetically silenced tumor antigens may induce an immunologically mediated antitumor response
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