M7318
Anti-MBD2a,b (RA-18) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-DMTase, Anti-NY-CO-41
Product Name
Anti-MBD2a,b (RA-18) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, human
technique(s)
indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells
microarray: suitable
western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MBD2(8932)
mouse ... Mbd2(17191)
Related Categories
Application
Anti-MBD2a,b (RA-18) antibody produced in rabbit has been used:
- for chromatin immunoprecipitation and western blotting
- immunofluorescence
- immunoblotting
Biochem/physiol Actions
MBD2 binds to methylated DNA in vitro or in vivo, suggesting that MBD2 targets the MeCP1 complex to methylated DNA. MBD2a interacts with RNA Helicase A (RHA). MBD2 protein appear to protect against some cancers.
Methyl-CpG binding domain protein 2 (MBD2a) acts as a repressor during transcription.
The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-MBD2a,b (RA-18) is developed in rabbit using as immunogen a synthetic peptide corresponding human MBD2a conjugated to KLH. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene; MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a is a component of the MeCP1 corepressor complex, which is a 400-800 kDa complex containing as components MBD2, Mi-2, MTA2, MBD3, and HDAC1/2.
The gene encoding methyl-CpG binding domain protein 2 (MBD2a) is localized on chromosome 18.
Immunogen
synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Ukrae H Cho et al.
eLife, 12 (2023-09-04)
During apoptosis, caspases degrade 8 out of ~30 nucleoporins to irreversibly demolish the nuclear pore complex. However, for poorly understood reasons, caspases are also activated during cell differentiation. Here, we show that sublethal activation of caspases during myogenesis results in
Jin Young Kim et al.
Molecular cell, 56(6), 738-748 (2014-12-03)
Mammalian circadian rhythms are generated by a negative feedback loop in which PERIOD (PER) proteins accumulate, form a large nuclear complex (PER complex), and bind the transcription factor CLOCK-BMAL1, repressing their own expression. We found that mouse PER complexes include
Nina Schmidt et al.
BMC cancer, 16, 399-399 (2016-07-09)
Increased numbers and improperly positioned centrosomes, aneuploidy or polyploidy, and chromosomal instability are frequently observed characteristics of cancer cells. While some aspects of these events and the checkpoint mechanisms are well studied, not all players have yet been identified. As
Antithetic effects of MBD2a on gene regulation
Fujita H, et al.
Molecular and cellular biology, 23(8), 2645-2657 (2003)
Pablo Gómez-Del Arco et al.
Cell metabolism, 23(5), 881-892 (2016-05-12)
Heart muscle maintains blood circulation, while skeletal muscle powers skeletal movement. Despite having similar myofibrilar sarcomeric structures, these striated muscles differentially express specific sarcomere components to meet their distinct contractile requirements. The mechanism responsible is still unclear. We show here
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