M7403
MCDB 153 Medium
With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Molecular, Cellular, and Development Biology 153 medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)
shipped in
ambient
storage temp.
2-8°C
General description
MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.
Application
MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.
Quantity
Formulated to contain 17.6 grams of powder per liter of medium.
Reconstitution
Supplement with 1.18 g/L sodium bicarbonate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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