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M8042

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Product Name

Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Hanks’ salts (2% CO₂): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO₂): yes
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Minimum Essential Medium (MEM)

Molecular Biology & Functional Genomics

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

Application

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.

Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Biochem/physiol Actions

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO₃, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.

Preparation Note

Supplement with 0.292 g/L L-glutamine

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone.

Eva Schmidt et al.

BMC research notes, 4, 282-282 (2011-08-13)

The tetracycline-controlled transactivator system is a powerful tool to control gene expression in vitro and to generate consistent and conditional transgenic in vivo model organisms. It has been widely used to study gene function and to explore pathological mechanisms involved

The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.

L Keay

Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)

The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved

Isolation of adult stem cells and their differentiation to Schwann cells

Progenitor Cells, 47-57 (2012)

A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone

Schmidt E and Eriksson M

BMC Research Notes, 4(1), 282-282 (2011)

Polymorphonuclear leukocytes isolated from umbilical cord blood as a useful research tool to study adherence to cell monolayers.

Janhavi Sharma et al.

Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)

One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase

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Global Trade Item Number

SKU	GTIN
M8042-500ML	04061834062646
M8042-6X500ML	04061834062653
M8042-24X500ML	04061834062639

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