Key Documents

View All Documentation

M8403

McCoy’s 5A Medium

Name: McCoy’s 5A Medium
Brand: SIGMA

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

5A basal medium

Select a Size

Change View

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

EC Number:

200-655-4

MDL number:

MFCD00217560

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Product Name

McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO₃: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Molecular Biology & Functional Genomics

General description

McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used to maintain:

G-361 melanoma cells;
87-MG cells
human colon carcinoma cell line HCT116

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Supplement with 0.22 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动物来源培养基

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The CEACAM1 tumor suppressor is an ATM and p53-regulated gene required for the induction of cellular senescence by DNA damage.

A-P Sappino et al.

Oncogenesis, 1, e7-e7 (2012-01-01)

The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily

Quantification of nanoparticle dose and vesicular inheritance in proliferating cells.

Huw D Summers et al.

ACS nano, 7(7), 6129-6137 (2013-06-19)

Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can

Fragment-Based Design of a Potent MAT2a Inhibitor and in Vivo Evaluation in an MTAP Null Xenograft Model.

Claudia De Fusco et al.

Journal of medicinal chemistry, 64(10), 6814-6826 (2021-04-27)

MAT2a is a methionine adenosyltransferase that synthesizes the essential metabolite S-adenosylmethionine (SAM) from methionine and ATP. Tumors bearing the co-deletion of p16 and MTAP genes have been shown to be sensitive to MAT2a inhibition, making it an attractive target for

Suppression of metastasis by mirtazapine via restoration of the Lin-7C/β-catenin pathway in human cancer cells.

Katsuhiro Uzawa et al.

Scientific reports, 4, 5433-5433 (2014-06-26)

No definitive therapy exists to treat human metastatic tumors. We reported previously that down-regulation of Lin-7C is essential for metastasis of human squamous cell carcinomas (hSCCs). In this study, we investigated the chemical restoration of Lin-7C expression and demonstrated its

Versatile and High-throughput Force Measurement Platform for Dorsal Cell Mechanics.

Seungman Park et al.

Scientific reports, 9(1), 13286-13286 (2019-09-19)

We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted

View All Related Papers

Articles

McCoy's 5A Modified Media Formulation

Global Trade Item Number

SKU	GTIN
M8403-24X500ML	04061834063049
M8403-500ML	04061837847417
M8403-6X500ML	04061834063056

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service