M8537
MCDB 131 Medium
With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
concentration
11.6 g/L
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
General description
MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP).
Application
MCDB-131 Medium has been used:
- as a component of the basal medium to culture bovine retinal endothelial cells
- to culture bovine lung microvascular endothelial cells
- for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells
Preparation Note
Formulated to contain 11.6 grams of powder per liter of medium.
Supplement with 1.18 g/L sodium bicarbonate.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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