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M9004

Malic Dehydrogenase from bovine heart

Name: Malic Dehydrogenase from bovine heart
Brand: SIGMA

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonym(s):

L-Malate:NAD⁺ oxidoreductase, MDH

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-622-5

MDL number:

MFCD00131567

EC Number:

1.1.1.37 (BRENDA, IUBMB)

Specific activity:

2000-4000 units/mg protein (modified Warburg-Christian)

Biological source:

bovine heart

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Properties

biological source

bovine heart

Quality Segment

200

form

ammonium sulfate suspension

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

mol wt

35 kDa

impurities

≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase

storage temp.

2-8°C

Description

General description

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Physical form

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Protocols

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

Global Trade Item Number

SKU	GTIN
M9004-5KU	04061834063797
M9004-10KU	04061834063780
M9004-25KU	04061832731001