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M9170

Sigma-Aldrich

Macrophage Colony-Stimulating Factor from mouse

M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
mCSF-1, mM-CSF, CSF-1, M-CSF
CAS Number:
81627-83-0
EC Number:
214-684-5
MDL number:
MFCD00166591
eCl@ss:
32190102

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

0.500-3.000 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 18.2 kDa (containing 156 amino acid residues)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

P07141

storage temp.

−20°C

Gene Information

mouse ... Csf1(12977)

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Application

Macrophage Colony-Stimulating Factor from mouse has been used:
  • in the initiation of osteoclastogenesis in bone marrow monocyte cells
  • to stimulate osteoclast differentiation in non-adherent hematopoietic cell
  • for the generation of blood-derived stem cells

Biochem/physiol Actions

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

Physical form

Lyophilized from a sterile filtered buffered aqueous solution.

Analysis Note

The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319 - H335

Precautionary Statements

P261 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Strain differences in the attenuation of bone accrual in a young growing mouse model of insulin resistance
Rendina-Ruedy E, et al.
Journal of Bone and Mineral Metabolism, 34(4), 380-394 (2016)
Yan Li et al.
Journal of immunology (Baltimore, Md. : 1950), 191(6), 3192-3199 (2013-08-13)
Engraftment of human CD34⁺ hematopoietic stem/progenitor cells into immunodeficient mice leads to robust reconstitution of human T and B cells but not monocytes and macrophages. To identify the cause underlying the poor monocyte and macrophage reconstitution, we analyzed human myeloid
Verena Turco et al.
Nature communications, 14(1), 771-771 (2023-02-12)
Glioblastoma, the most common and aggressive primary brain tumor type, is considered an immunologically "cold" tumor with sparse infiltration by adaptive immune cells. Immunosuppressive tumor-associated myeloid cells are drivers of tumor progression. Therefore, targeting and reprogramming intratumoral myeloid cells is
B F Boyce
Journal of dental research, 92(10), 860-867 (2013-08-03)
Osteoclasts are derived from mononuclear hematopoietic myeloid lineage cells, which are formed in the bone marrow and are attracted to the bloodstream by factors, including sphingsine-1 phosphate. These circulating precursors are attracted to bone surfaces undergoing resorption by chemokines and
Filamin A regulates monocyte migration through Rho small GTPases during osteoclastogenesis
Leung R, et al.
Journal of Bone and Mineral Research, 25(5), 1077-1091 (2010)
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