M9170
Macrophage Colony-Stimulating Factor from mouse
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
mCSF-1, mM-CSF, CSF-1, M-CSF
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About This Item
CAS Number:
UNSPSC Code:
12352202
eCl@ss:
32190102
EC Number:
214-684-5
NACRES:
NA.77
MDL number:
Biological source:
mouse
Recombinant:
expressed in E. coli
Assay:
≥98% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
dimer 18.2 kDa (containing 156 amino acid residues)
Impurities:
≤1.000 EU/μg
Product Name
Macrophage Colony-Stimulating Factor from mouse, M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
mouse
Quality Level
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
≤3.000 ng/mL Suitability ED50
quality
endotoxin tested
mol wt
dimer 18.2 kDa (containing 156 amino acid residues)
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1.000 EU/μg
color
white
UniProt accession no.
storage temp.
−20°C
Application
Macrophage Colony-Stimulating Factor from mouse has been used:
- in the initiation of osteoclastogenesis in bone marrow monocyte cells
- to stimulate osteoclast differentiation in non-adherent hematopoietic cell
- for the generation of blood-derived stem cells
Biochem/physiol Actions
Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.
Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF augments many of the physiological functions of macrophages, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing and glycosylation the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.
Physical form
Lyophilized from a sterile filtered buffered aqueous solution.
Analysis Note
The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Glioblastoma, the most common and aggressive primary brain tumor type, is considered an immunologically "cold" tumor with sparse infiltration by adaptive immune cells. Immunosuppressive tumor-associated myeloid cells are drivers of tumor progression. Therefore, targeting and reprogramming intratumoral myeloid cells is
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Journal of immunology (Baltimore, Md. : 1950), 191(6), 3192-3199 (2013-08-13)
Engraftment of human CD34⁺ hematopoietic stem/progenitor cells into immunodeficient mice leads to robust reconstitution of human T and B cells but not monocytes and macrophages. To identify the cause underlying the poor monocyte and macrophage reconstitution, we analyzed human myeloid
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The colony-stimulating factor 1 receptor (CSF1R) is a key regulator of myeloid lineage cells. Genetic loss of the CSF1R blocks the normal population of resident microglia in the brain that originates from the yolk sac during early development. However, the
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M9170-10UG | 04061834064039 |
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