Anti-Macrophage Inflammatory Protein-2, Viral antibody produced in goat

The genome of Kaposi′s sarcoma-associated herpes virus (KSHV) encodes two gene products that are similar to the sequence of human chemokine, MIP-1α. These are named according to the human protein counterpart as viral macrophage inflammatory protein (vMIP)-I and –II. The sequence identity of vMIP-I and vMIP-II to human MIP-1α is 37.9% and 41.1%, respectively. The expression of vMIP-1 and vMIP-2 are reportedly induced by phorbol esters in latently infected lymphoma cells. Both vMIP-I and vMIP-II partially inhibit the infection of peripheral blood mononuclear cells by HIV. vMIP-II also reportedly blocks the infection of CD4-positive cell line expressing CCR3 by HIV, type-1
Anti-Macrophage Inflammatory Protein-II (MIP-II) recognizes recombinant viral MIP-II. The antibody shows less than 10% cross reactivity with recombinant viral MIP-I and recombinant human MIP-1b.

recombinant viral MIP 2, expressed in E. coli.

Anti-Macrophage Inflammatory Protein-II (MIP-II) antibody may be used in immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA the recommended concentration is 0.5-1.0 μg/ml.

The antibody will recognize recombinant viral MIP-2. The antibody shows <10% cross-reactivity with recombinant viral MIP-1 and recombinant human MIP-1β.

Lyophilized from a 0.2 μm-filtered solution in phosphate-buffered saline.