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M9763

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate

fluorogenic, ≥98% (TLC), powder

Synonym(s):

4-Methylumbelliferyl-N,N′-diacetyl-β-D-chitobioside hydrate

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About This Item

Empirical Formula (Hill Notation):
C26H34N2O13 · xH2O
CAS Number:
53643-12-2
Molecular Weight:
582.55 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
24897364
NACRES:
NA.28
MDL number:
MFCD00082499
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Product Name

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate, ≥98% (TLC)

Quality Segment

assay

≥98% (TLC)

form

powder

solubility

DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow

fluorescence

λex 330 nm; λem 375 nm in methanol

storage temp.

−20°C

SMILES string

CC(=O)NC1C(O)C(O)C(CO)OC1OC2C(O)C(NC(C)=O)C(OC2CO)Oc3ccc4C(C)=CC(=O)Oc4c3

InChI

1S/C26H34N2O13/c1-10-6-18(33)38-15-7-13(4-5-14(10)15)37-25-20(28-12(3)32)23(36)24(17(9-30)40-25)41-26-19(27-11(2)31)22(35)21(34)16(8-29)39-26/h4-7,16-17,19-26,29-30,34-36H,8-9H2,1-3H3,(H,27,31)(H,28,32)

InChI key

UPSFMJHZUCSEHU-UHFFFAOYSA-N

Application

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate has been used as a fluorogenic substrate:
  • in chitinase enzyme assay of bronchoalveolar lavage samples
  • in acidic mammalian chitinase activity assay of human gastric juice
  • in chitinase assay in crystalline style extracts of Greenshell Mussels and fungus Fusarium oxysporum.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate (MUF-diNAG) is an analog of chitin. It is a fluorogenic substrate for chitin depolymerase and its breakdown results in methylumbelliferone, a fluorescent product.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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