Skip to Content
Merck
CN

M9763

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate

fluorogenic, ≥98% (TLC), powder

Synonym(s):

4-Methylumbelliferyl-N,N′-diacetyl-β-D-chitobioside hydrate

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C26H34N2O13 · xH2O
CAS Number:
53643-12-2
Molecular Weight:
582.55 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
24897364
NACRES:
NA.28
MDL number:
MFCD00082499

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate, ≥98% (TLC)

SMILES string

CC(=O)NC1C(O)C(O)C(CO)OC1OC2C(O)C(NC(C)=O)C(OC2CO)Oc3ccc4C(C)=CC(=O)Oc4c3

InChI key

UPSFMJHZUCSEHU-UHFFFAOYSA-N

InChI

1S/C26H34N2O13/c1-10-6-18(33)38-15-7-13(4-5-14(10)15)37-25-20(28-12(3)32)23(36)24(17(9-30)40-25)41-26-19(27-11(2)31)22(35)21(34)16(8-29)39-26/h4-7,16-17,19-26,29-30,34-36H,8-9H2,1-3H3,(H,27,31)(H,28,32)

assay

≥98% (TLC)

form

powder

solubility

DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow

fluorescence

λex 330 nm; λem 375 nm in methanol

storage temp.

−20°C

Quality Level

200

Application

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate has been used as a fluorogenic substrate:
  • in chitinase enzyme assay of bronchoalveolar lavage samples
  • in acidic mammalian chitinase activity assay of human gastric juice
  • in chitinase assay in crystalline style extracts of Greenshell Mussels and fungus Fusarium oxysporum.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate (MUF-diNAG) is an analog of chitin. It is a fluorogenic substrate for chitin depolymerase and its breakdown results in methylumbelliferone, a fluorescent product.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
13190416
0000308064
0000486501
0000486500
0000486500

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Polysaccharidases in the crystalline styles of selectively bred Greenshell mussel (Perna canaliculus Gmelin) families
Mackenzie AL and Marshall C
Journal of shellfish research., 33(3), 687-694 (2014)
Functional comparison of recombinant acidic mammalian chitinase with enzyme from murine bronchoalveolar lavage
Goedken ER, et al.
Protein Expression and Purification, 75(1), 55-62 (2011)
Quantification of Chitinase Activity in Fusarium oxysporum
Ruiz-Roldan C and Roncero MIG
Bio-protocol, 6(15) (2016)
Human gastric juice contains chitinase that can degrade chitin
Paoletti MG, et al.
Annals of Nutrition & Metabolism, 51(3), 244-251 (2007)
Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40
Howard MB, et al.
Journal of Bacteriology, 185(11), 3352-3360 (2003)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service