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M9901

Mutanolysin from Streptomyces globisporus ATCC 21553

lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified

Synonym(s):

Mutanolysin

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About This Item

CAS Number:
55466-22-3
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00131635
Specific activity:
≥4000 units/mg protein (biuret)
Biological source:
bacterial (Streptomyces globisporus)
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biological source

bacterial (Streptomyces globisporus)

Quality Level

300

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

Application

Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Other Notes

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

Ficoll is a registered trademark of Cytiva

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000546477
0000546479
0000546476
0000546478
0000546475

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Pierre Godessart et al.
Nature microbiology, 6(1), 27-33 (2020-11-04)
Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Amelia T Soderholm et al.
Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)
Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host
Helena Žemličková et al.
Journal of medical microbiology, 67(7), 1003-1011 (2018-06-02)
Purpose. The aim of this study was to characterize serogroup 19 isolates resistant to macrolides and/or penicillin found among pneumococci recovered from cases of invasive and respiratory tract disease in the Czech Republic in 2014.Methods. Pneumococcal isolates of serotypes 19A
Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci
Kondo, J. and L. McKay
Journal of Dairy Science, 65, 1428-1431 (1982)
Matthew J Sullivan et al.
Applied and environmental microbiology, 84(18) (2018-07-15)
Group B streptococcus (GBS) is a Gram-positive bacterium associated with various diseases in humans and animals. Many studies have examined GBS physiology, virulence, and microbe-host interactions using diverse imaging approaches, including fluorescence microscopy. Strategies to label and visualize GBS using
View All Related Papers

Protocols

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

GenElute™细菌基因组DNA试剂盒实验方案（NA2100、NA2110、NA2120）

的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。

变溶菌素的酶促检测

在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。

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Product Information Sheet - M9901
Complex Carbohydrate Analysis- Enzymes, Kits and Reagents

Global Trade Item Number

SKUGTIN
M9901-1KU04061835557806
M9901-10KU04061835557790
M9901-50KU04061835557813
M9901-5KU04061835557820

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