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Merck
CN

MAK044

Free Fatty Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Free Fatty Acid Assay Kit

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About This Item

UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

Free fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.

Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid β-oxidation is implicated in cardiac ischemia.

Application

Free Fatty Acid Quantitation Kit has been used in the following:
  • to measure lipid hydrolysis, by determining the free fatty acid in digested sample.
  • to measure plasma and serum free fatty acids level.
  • to quantify free fatty acids level in liver tissue.
Suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.

Biochem/physiol Actions

In this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the fatty acids present.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Regulatory Information

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Association of Temperament and acute stress responsiveness with Productivity, Feed efficiency, and Methane emissions in Beef cattle: an Observational study
Llonch P, et al.
Frontiers in veterinary science, 3, 43-43 (2016)
Sarmistha Mukherjee et al.
Hepatology (Baltimore, Md.), 65(2), 616-630 (2016-11-04)
The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of nicotinamide adenine dinucleotide (NAD) falls, at least in part due to metabolic competition for
Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy.
Chiu H C, et al.
Circulation Research, 96(2), 225-233 (2005)
Interactions among macronutrients in wheat flour determine their enzymic susceptibility
Bhattarai R R, et al.
Food Hydrocolloids, 61, 415-425 (2016)
Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression.
A S M H Mahmood et al.
ASN neuro, 12, 1759091420910933-1759091420910933 (2020-04-03)

Related Content

Global Trade Item Number

SKUGTIN
MAK044-1KT04061835546954

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