MAK051
Aconitase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Aconitase Enzyme Activity Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
Aconitase Activity Assay Kit, sufficient for 100 colorimetric tests
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
neurological disorders; cardiovascular diseases; cancer
storage temp.
2-8°C
Gene Information
human ... ACO1(48), ACO2(50)
mouse ... ACO1(11428), ACO2(11429)
rat ... ACO1(50655), ACO2(79250)
Application
Aconitase Activity Assay Kit has been used to measure the activity of cytosolic and mitochondrial aconitase enzyme.
Suitable for the measurement of aconitase activity in biological samples including tissue and cells
Biochem/physiol Actions
The Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of aconitase is the amount of enzyme that will isomerize 1.0 μmole of citrate from isocitrate per minute at pH 7.4 at 25 °C.
Features and Benefits
Compatible with high-throughput handling systems.
General description
The aconitases are a family of iron-sulfur containing enzymes that catalyzes the isomerization of citrate to isocitrate. Two aconitases, encoded by different genes, have been identified in eukaryotes. Aconitase 1 (ACO1, c-aconitase) is a cytosolic enzyme while Aconitase 2 (ACO2, m-aconitase) is a mitochondrial enzyme that functions in the tricarboxylic acid cycle. Aconitases are reversibly inactivated by oxidative stress, and aconitase activity in cells and tissues has been used as a biomarker for oxidative damage.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
Regulatory Information
低风险生物材料
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NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis.
Alvarez S W, et al.
Nature, 551(7682), 639-639 (2017)
Spyridon Gourdoupis et al.
Journal of the American Chemical Society, 140(43), 14401-14412 (2018-10-03)
The maturation of mitochondrial iron-sulfur proteins requires a complex protein machinery. Human IBA57 protein was proposed to act in a late phase of this machinery, along with GLRX5, ISCA1, and ISCA2. However, a molecular picture on how these proteins cooperate
François Beaufay et al.
mBio, 11(4) (2020-07-30)
Maintaining cellular iron homeostasis is critical for organismal survival. Whereas iron depletion negatively affects the many metabolic pathways that depend on the activity of iron-containing enzymes, any excess of iron can cause the rapid formation of highly toxic reactive oxygen
Vincenzo Maione et al.
Biochimica et biophysica acta. General subjects, 1862(9), 1980-1987 (2018-05-31)
The CIA2A protein, in complex with CIAO1, has been proposed to be exclusively implicated in the maturation of cytosolic aconitase. However, how the CIA2A-CIAO1 complex generates active aconitase is still unknown and the available structural information has not provided any
Jayasimman Rajendran et al.
EMBO molecular medicine, 11(1) (2018-12-12)
Alternative oxidase (AOX) is a non-mammalian enzyme that can bypass blockade of the complex III-IV segment of the respiratory chain (RC). We crossed a Ciona intestinalis AOX transgene into RC complex III (cIII)-deficient Bcs1l
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