Merck
CN
All Photos(3)

Documents

Safety Information

MAK052

Sigma-Aldrich

ALT Activity Assay

sufficient for 100 colorimetric or fluorometric tests

Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Alanine Aminotransferase Activity Assay Kit, SGPT Activity Assay Kit, Serum Glutamic-Pyruvic Transaminase Activity Assay Kit
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)

Related Categories

General description

Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as well. Hepatocellular injury often results in an increase of serum ALT levels and serum ALT levels can be used as a marker for liver injury.

Application

ALT Activity Assay kit has been used to determine the activity of alanine aminotransferase (ALT) in serum samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of alanine aminotransferase (ALT) activity in biological samples including tissue, cells and serum.

Principle

The ALT Activity Assay Kit provides a simple and direct procedure for measuring ALT activity in a variety of biological samples.

ALT activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate generated. One unit of ALT is defined as the amount of enzyme that generates 1.0 μmole of pyruvate per minute at 37 °C.

related product

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334 - H412

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Brett Palaschak et al.
Molecular therapy. Methods & clinical development, 5, 142-152 (2017-05-10)
Multiple independent adeno-associated virus (AAV) gene therapy clinical trials for hemophilia B, utilizing different AAV serotypes, have reported a vector dose-dependent loss of circulating factor IX (FIX) protein associated with capsid-specific CD8
Investigation of Therapeutic Effects of $\alpha$-Mangostin on Thioacetamide-Induced Cirrhosis in Rats
Nilbunga S.
Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 98(9), S91-S97 (2015)
L Bočkor et al.
Gene therapy, 24(10), 649-660 (2017-08-15)
Adeno-associated virus (AAV) -mediated gene therapy is a promising strategy to treat liver-based monogenic diseases. However, two major obstacles limit its success: first, vector dilution in actively dividing cells, such as hepatocytes in neonates/children, due to the non-integrating nature of
Resolvin D1 and E1 alleviate the progress of hepatitis toward liver cancer in long-term concanavalin A-induced mice through inhibition of NF-KB activity.
Kuang H, et al.
Oncology Reports, 35(1), 307-317 (2016)
Nan Wu et al.
Laboratory investigation; a journal of technical methods and pathology, 97(7), 843-853 (2017-06-06)
The Hippo signaling pathway and the Notch signaling pathway are evolutionary conserved signaling cascades that have important roles in embryonic development of many organs. In murine liver, disruption of either pathway impairs intrahepatic bile duct development. Recent studies suggested that
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service