Glucose-1-Phosphate (G1P) Colorimetric Assay Kit

Glucose-1-phosphate (G1P), also known as the Cori ester due to its discovery by Gerty and Carl Cori, is produced from glycogen during glycogenolysis by the actions of glycogen phosphorylase. Conversion to glucose-6-phosphate (G6P) by phosphoglucomutase allows for entry of the glucose molecule into metabolic pathways such as glycolysis. During glycogenesis, G6P is converted to G1P by the actions of phosphoglucose isomerase.

Suitable for the measurement of glucose-1-phosphate in various tissues (liver, muscle and heart etc.) and cells (adherent or suspension), the analysis of glucose metabolism and cell signaling in various cells and screening anti-diabetic drugs.

The G1P Colorimetric Assay kit provides a simple and direct procedure for measuring G1P in a variety of samples. G1P concentration is determined by a coupled enzyme assay, in which G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of glucose-1,6-biphosphate. The glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the G1P present.