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Merck
CN

MAK100

Inosine Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Inosine Fluorometric Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

cardiovascular diseases; immunological diseases

storage temp.

−20°C

General description

Inosine is a purine nucleotide found at the wobble position of tRNA where it plays a role in the proper translation of mRNA at the ribosome. In addition to its role in translation, inosine also plays important roles in the immune system where is exhibits both inflammatory and anti-inflammatory effects. Inosine may be tissue protective during ischemic injury and recent reports suggest inosine may preserve cell viability during hypoxia.

Application

Inosine Assay Kit has been used for the detection of inosine in the brain tissue homogenates.
Suitable for the measurement of inosine in a variety of samples including tissue and cells

Biochem/physiol Actions

The Inosine Assay Kit provides a simple and direct procedure for measuring inosine in a variety of samples. Inosine concentration is determined by a coupled enzyme reaction in which inosine is converted to hypoxanthine which reacts with the substrate mix and probe, resulting in a fluorometric product (λex = 535/λem = 587 nm), proportional to the inosine present.


Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3



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