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Merck
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MAK117

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

Key Documents

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Product Name

Glycerol Assay Kit, sufficient for 200 colorimetric or fluorometric tests

usage

sufficient for 200 colorimetric or fluorometric tests

input

food(s)
beverage(s)
biological sample(s)

species reactivity

Drosophila, human, mouse

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

activity assay: suitable

accession no.

NM_000167.6
NM_001273184.2
P13706
P32189

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

Gene Information

Drosophila ... Gpdh1(33824)
human ... GK(2710 )
mouse ... Gpd1(14555)

Related Categories

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.
This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Biochem/physiol Actions

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Features and Benefits

Compatible with high-throughput handling systems.

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Storage Class

10 - Combustible liquids

wgk

WGK 2

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
117CF08A22
117CF08A22
117CF07A09
117CF07A09
117CF04A04

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