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Merck
CN

MAK117

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 200 colorimetric or fluorometric tests

input

food(s)
beverage(s)
biological sample(s)

species reactivity

Drosophila, human, mouse

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

activity assay: suitable

accession no.

NM_000167.6, NM_001273184.2, P13706, P32189

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

Gene Information

Drosophila ... Gpdh1(33824)
human ... GK(2710 )
mouse ... Gpd1(14555)

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.
This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Biochem/physiol Actions

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Features and Benefits

Compatible with high-throughput handling systems.

Storage Class

10 - Combustible liquids

wgk

WGK 2

Regulatory Information

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Sylvia J Gasparini et al.
Diabetologia, 62(8), 1463-1477 (2019-05-18)
Chronic glucocorticoid therapy causes insulin resistance, dyslipidaemia, abnormal fat accumulation, loss of muscle mass and osteoporosis. Here we describe a hitherto unknown sexual dimorphism in the metabolic response to chronic glucocorticoid exposure in mice. This led us to investigate whether
Jessica C Brooks et al.
The Analyst, 141(20), 5714-5721 (2016-08-04)
Employing 3D-printed templates for macro-to-micro interfacing, a passively operated polydimethysiloxane (PDMS) microfluidic device was designed for time-resolved secretion sampling from primary murine islets and epidiymal white adipose tissue explants. Interfacing in similar devices is typically accomplished through manually punched or
Nicole E Nozzi et al.
ACS synthetic biology, 6(11), 2136-2144 (2017-07-19)
Cyanobacteria have attracted significant interest as a platform for renewable production of fuel and feedstock chemicals from abundant atmospheric carbon dioxide by way of photosynthesis. While great strides have been made in developing this technology in freshwater cyanobacteria, logistical issues
Martina Rudnicki et al.
Frontiers in physiology, 9, 1452-1452 (2018-11-09)
Background: Impaired capillary growth (angiogenesis) in skeletal muscle and adipose tissue contributes to the development of metabolic disorders in obese males. This association remains unexplored in females, despite mounting evidence that endothelial cells have sex-specific transcriptional profiles. Therefore, herein we
Paula Dunayevich et al.
Scientific reports, 8(1), 15168-15168 (2018-10-13)
Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general

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