MAK120
Urease Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Urea Amidohydrolase Activity Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric tests
application(s)
environmental
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
2-8°C
General description
Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.
Application
Suitable for the detection of Urease activity in biological and environmental samples.
Urease Activity Assay Kit has been used to measure urease activity.
Biochem/physiol Actions
In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B
supp_hazards
Storage Class
8B - Non-combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Instructions
Ureases I. Functional, catalytic and kinetic properties: A review
Barbara K.
Journal of Molecular Catalysis. B, Enzymatic, 59(1-3), 9-21 (2009)
Chelsea M Heveran et al.
Scientific reports, 9(1), 14721-14721 (2019-10-13)
We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO3) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO3. However, control
Ah-Young Jee et al.
Journal of the American Chemical Society, 141(51), 20062-20068 (2019-11-30)
The concept that catalytic enzymes can act as molecular machines transducing chemical activity into motion has conceptual and experimental support, but experimental support has involved oligomeric enzymes, often studied under conditions where the substrate concentration is higher than biologically relevant
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAK120-1KT | 04061834065418 |