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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
neurological disorders; gastrointestinal diseases; hematological disorder
storage temp.
−20°C
Gene Information
human ... GBA(2629), GBA2(57704), GBA3(57733)
mouse ... GBA(14466), GBA2(230101)
rat ... GBA(684536), GBA2(298399), GBA3(289687)
Related Categories
General description
β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.
Application
β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.
The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.
Biochem/physiol Actions
In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.
Features and Benefits
Compatible with high-throughput handling systems.
Storage Class Code
12 - Non Combustible Liquids
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