MAK134
Ketone Body Assay
sufficient for 200 tests (UV)
Synonym(s):
Ketone Quantification Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
Ketone Body Assay, sufficient for 200 tests (UV)
usage
sufficient for 200 tests (UV)
feature
This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer
storage temp.
−20°C
Related Categories
Application
Ketone Body Assay has been used to measure the release of ketone bodies in the human liver cancer cell line HepG2 culture medium.
Suitable for the detection of ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) in serum, plasma, urine, and other biological samples.
Biochem/physiol Actions
In this kit, AcAc and BOH levels are determined using an enzymatic assay based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance measured at 340 nm is directly related to either the AcAc and BOH concentrations.
Features and Benefits
Compatible with high-throughput handling systems
General description
Ketone bodies, 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc), are produced in the liver primarily from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism.
Storage Class
10 - Combustible liquids
Regulatory Information
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Kim J Shimy et al.
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Kanikarla M, et al.
Free Radical Biology & Medicine, 95, 268-277 (2016)
Zachary M Augur et al.
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Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease.
Dedkova E N and Lothar A B
Frontiers in Physiology, 5, 260-260 (2014)
Anila K Madiraju et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(24), E3423-E3430 (2016-06-02)
A key sensor of cellular energy status, AMP-activated protein kinase (AMPK), interacts allosterically with AMP to maintain an active state. When active, AMPK triggers a metabolic switch, decreasing the activity of anabolic pathways and enhancing catabolic processes such as lipid
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