MAK162
Colorimetric Maleimide Assay Kit
sufficient for 100 colorimetric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
−20°C
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General description
Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.
Application
Suitable for quantifying maleimide groups
Biochem/physiol Actions
The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Carbon Composites: Composites with Carbon Fibers, Nanofibers, and Nanotubes (2016)
Efficient site-specific labeling of proteins via cysteines.
Kim Y, et al.
Bioconjugate Chemistry, 19(3), 786-791 (2008)
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