MAK168
Pyrophosphate Assay Kit
sufficient for 200 fluorometric tests (Blue fluorescence)
Synonym(s):
High-Sensitivity Pyrophosphate Assay Kit
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84
usage
sufficient for 200 fluorometric tests (Blue fluorescence)
application(s)
cosmetics
food and beverages
detection method
fluorometric
storage temp.
−20°C
General description
Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.
Application
The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.
Biochem/physiol Actions
The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =316/λem = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F
flash_point_c
87 °C
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Instructions
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Kottur J and Deepak T N
Nucleic Acids Research (2018)
Peiyi Zheng et al.
Journal of molecular biology, 431(4), 764-776 (2019-01-18)
Phosphopantothenoylcysteine (PPC) synthetase (PPCS) catalyzes nucleoside triphosphate-dependent condensation reaction between 4'-phosphopantothenate (PPA) and l-cysteine to form PPC in CoA biosynthesis. The catalytic mechanism of PPCS has not been resolved yet. Coenzyme A biosynthesis protein 2 (Cab2) possesses activity of PPCS
Jithesh Kottur et al.
Nucleic acids research, 46(12), 5875-5885 (2018-06-01)
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAK168-1KT | 04061834065630 |