High Sensitivity Pyrophosphate Assay Kit

Name: High Sensitivity Pyrophosphate Assay Kit
Brand: SIGMA

sufficient for 200 fluorometric tests

Synonym(s):

Pyrophosphate (PPi) Assay Kit

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About This Item

UNSPSC Code:

12161503

EC Number:

200-664-3

NACRES:

NA.84

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Properties

usage

sufficient for 200 fluorometric tests

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

genitourinary disorders; rheumatological diseases

storage temp.

−20°C

Description

General description

Pyrophosphate (PP_i) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.

Application

The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.

Biochem/physiol Actions

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λ_ex =370/λ_em = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.