MAK185
Urokinase Activity Fluorometric Assay Kit
Sufficient for 100 Fluorometric tests
Synonym(s):
Plasminogen Activity Assay Kit, uPA Activity Assay Kit
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... PLAUH(5328)
mouse ... PLAUH(18792)
rat ... PLAUH(25619)
General description
Urokinase (Urokinase-type plasminogen activator, uPA) is a serine protease that catalyzes the conversion of plasminogen to active plasmin. Urokinase is involved in diverse cellular processes including blood coagulation, cell adhesion, fibrinolysis, and cell migration. Studies in cultured fibroblasts have revealed urokinase induces MMP-9 expression and reactive oxygen species (ROS) generation. Suppression of uPA expression has been studied for therapeutic implications in hepatocellular carcinoma (HCC). Urokinase activity measurements may be useful for the study of cell functions and cancer therapy.
Application
Suitable for the detection of Urokinase Activity in variety of samples such as serum, cell and nuclear extracts, and with purified enzymes.
Biochem/physiol Actions
The Urokinase Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring urokinase activity (ranging from 0.01-0.5 IU/well) in a variety of samples. Urokinase activity is determined using the enzymatic cleavage of an AMC-based (amido-methylcoumarin) peptide substrate, which results in the generation of AMC (λex = 350/λem = 450 nm) proportional to the enzymatic activity present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Regulatory Information
高风险级别生物产品--人源产品
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