MAK190
ATP Assay Kit
Sufficient for 100 Colorimetric or Fluorometric tests
Synonym(s):
Adenosine triphosphate Assay Kit
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About This Item
UNSPSC Code:
12161503
EC Number:
213-579-1
NACRES:
NA.84
detection method
colorimetric, fluorometric
relevant disease(s)
cancer; neurological disorders
storage temp.
−20°C
General description
Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.
Application
ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.
Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.
Biochem/physiol Actions
The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.
Other Notes
The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.
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Kit Components Only
Product No.
Description
- ATP Assay Buffer
- ATP Probe, in DMSO
- ATP Converter
- Developer Mix
- ATP Standard, 1.0 μmole
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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