Skip to Content
Merck
CN

MAK196

Malate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

MDH Activity Assay Kit

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... MDH1(4190), MDH1B(130752), MDH2(4191)
mouse ... MDH1(17449), MDH1B(76668), MDH2(17448)
rat ... MDH1(24551), MDH1B(316444), MDH2(81829)

Looking for similar products? Visit Product Comparison Guide

General description

Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.

Application

Malate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays. It has also been used to estimate isocitrate dehydrogenase and succinate dehydrogenase activities.
Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Biochem/physiol Actions

The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.

Kit Components Only

Product No.
Description
  • MDH Assay Buffer
  • MDH Substrate
  • MDH Enzyme Mix
  • MDH Developer
  • NADH Standard
  • MDH Positive Control

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

Regulatory Information

低风险生物材料
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
5J09K06540
7J16K06540
2L02K06540
B9K110654
B9K050654

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers.
Mullarky E, et al.
Proceedings of the National Academy of Sciences of the USA, 113(7), 1778-1783 (2016)
Nan Nan et al.
Plant biotechnology journal, 18(1), 172-184 (2019-06-05)
Salinity is an important environmental factor that adversely impacts crop growth and productivity. Malate dehydrogenases (MDHs) catalyse the reversible interconversion of malate and oxaloacetate using NAD(H)/NADP(H) as a cofactor and regulate plant development and abiotic stress tolerance. Vitamin B6 functions
Jinliang Wang et al.
Scientific reports, 9(1), 12300-12300 (2019-08-25)
The human mitochondrial heat shock protein 60 (hsp60) is a tetradecameric chaperonin that folds proteins in the mitochondrial matrix. An hsp60 D3G mutation leads to MitCHAP-60, an early onset neurodegenerative disease while hsp60 V72I has been linked to SPG13, a
Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.
Tripathi S K, et al.
The Journal of Biological Chemistry, jbc-M117 (2017)
Anna Antolak et al.
Journal of cellular biochemistry, 118(12), 4323-4330 (2017-04-22)
Drug dependence is an escalating problem worldwide and many efforts are being made to understand the molecular basis of addiction. The morphine model is widely used in these investigations. To date, at least 29 studies exploring the influence of morphine
View All Related Papers

Protocols

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

Related Content

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service