High Sensitivity β-Hydroxybutyrate Fluorometric Assay Kit

β -Hydroxybutyrate (β-HB), also known as 3-hydroxybutyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Ketone body formation, ketogenesis, is elevated when blood glucose levels drop. Moderately elevated blood ketone bodies occur normally during fasting and prolonged exercise. During alcoholic or diabetic ketoacidosis, blood levels of β -Hydroxybutyrate can rise to abnormally high levels. β -Hydroxybutyrate can be used as a marker for hepatic energy metabolism.

Suitable for use with serum, plasma, urine, and other biological fluids, as well as food products such as milk.

The High Sensitivity β-Hydroxybutyrate Assay Kit is a simple, sensitive, high-throughput adaptable method for quantifying β-hydroxybutyrate when sample quantities are limited or sub-ketogenic levels are suspected. In this assay, β-hydroxybutyrate is enzymatically oxidized generating a fluorometric signal (lex = 535 nm/lem = 587 nm), proportional to the amount of β-hydroxybutyrate present. The assay is sensitive to 4 mM of β-hydroxybutyrate in a variety of biological samples.