MAK277
GAPDH Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Glyceraldehyde-3-phosphate Dehydrogenase Activity Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
−20°C
General description
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.
Application
GAPDH Activity Assay Kit has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in testis.
Suitable for use with various tissue and cell culture samples.
Biochem/physiol Actions
The GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. GAPDH activity is determined in a coupled enzyme reaction in which GAP is converted to BPG by GAPDH. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. The assay is sensitive to 100 mUnits/mL. One unit of GAPDH activity is the amount of enzyme that will generate 1.0 mmole of NADH per minute at pH 7.2 at 37 °C.
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
Regulatory Information
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Samir El Qaidi et al.
Scientific reports, 11(1), 3834-3834 (2021-02-17)
Type III secretion system effector proteins have primarily been characterized for their interactions with host cell proteins and their ability to disrupt host signaling pathways. We are testing the hypothesis that some effectors are active within the bacterium, where they
Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1, 6-diphosphate.
May A L and Renno W M
PeerJ, 4, e2195-e2195 (2016)
May Al-Maghrebi et al.
PeerJ, 4, e2195-e2195 (2016-07-22)
Background. Testicular ischemia reperfusion injury (tIRI) is considered the mechanism underlying the pathology of testicular torsion and detorsion. Left untreated, tIRI can induce testis dysfunction, damage to spermatogenesis and possible infertility. In this study, we aimed to assess the activities
Catríona M Dowling et al.
Science advances, 7(3) (2021-02-02)
Triple-negative breast cancer (TNBC) is a subtype of breast cancer without a targeted form of therapy. Unfortunately, up to 70% of patients with TNBC develop resistance to treatment. A known contributor to chemoresistance is dysfunctional mitochondrial apoptosis signaling. We set
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