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MAK321

Sigma-Aldrich

Acetaldehyde Assay Kit

sufficient for 100 fluorometric tests

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usage

sufficient for 100 fluorometric tests

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

gastrointestinal diseases

storage temp.

−20°C

Related Categories

General description

Acetaldehyde is one of the most widely occurring aldehydes in nature and commonly used in industry. The metabolic byproduct of ethanol in the liver, acetaldehyde is toxic to the human body and rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing known as alcohol flush reaction. Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.

Application

The Acetaldehyde Assay Kit may be used for:
  • Food and Beverage Testing
  • Gastrointestinal Disease Research

Features and Benefits

Compatible with high-throughput handling systems.
Enhanced Detection Range: Detect Acetaldehyde levels accurately across a wide range, from 0.5 µM to 60 µM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice)

Principle

The fluorimetric acetaldehyde assay is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the generated NADH reduces a probe making it fluorescent. The fluorescence intensity of the product measured at λex/em = 530/585 nm is directly proportional to acetaldehyde concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H290 - H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Regulatory Information

含少量动物源组分生物产品
常规特殊物品

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Helmut K Seitz et al.
Genes & nutrition, 5(2), 121-128 (2009-10-23)
Chronic ethanol consumption is a strong risk factor for the development of certain types of cancer including those of the upper aerodigestive tract, the liver, the large intestine and the female breast. Multiple mechanisms are involved in alcohol-mediated carcinogenesis. Among
Dirk W Lachenmeier et al.
Addiction (Abingdon, England), 104(4), 533-550 (2009-04-02)
In addition to being produced in ethanol metabolism, acetaldehyde occurs naturally in alcoholic beverages. Limited epidemiological evidence points to acetaldehyde as an independent risk factor for cancer during alcohol consumption, in addition to the effects of ethanol. This study aims
Mikko Salaspuro
Journal of digestive diseases, 12(2), 51-59 (2011-03-16)
Aldehyde dehydrogenase (ALDH2) and alcohol dehydrogenase (ADH) gene polymorphisms associating with enhanced acetaldehyde exposure and markedly increased cancer risk in alcohol drinkers provide undisputable evidence for acetaldehyde being a local carcinogen not only in esophageal but also in gastric cancer.

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