MAK339
Lipopolysaccharide (LPS) Isolation Kit
sufficient for 10 isolation(s)
Synonym(s):
LPS Extraction Kit
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About This Item
UNSPSC Code:
12161503
usage
sufficient for 10 isolation(s)
application(s)
sample preservation
storage temp.
−20°C
General description
The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis.
Application
Suitable for the isolation of Lipopolysaccharides from outer membrane of gram negative bacteria.
Biochem/physiol Actions
The Lipopolysaccharide (LPS) Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chloroform or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified.
signalword
Danger
hcodes
Hazard Classifications
Met. Corr. 1 - Resp. Sens. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
10 - Combustible liquids
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