MAK348
Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Synonym(s):
Fluorometric Gelatinase Activity Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352200
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
General description
Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells.
Application
Suitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etc
Biochem/physiol Actions
The Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU).
Features and Benefits
Compatible with high-throughput handling systems.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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