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MAK440

Sigma-Aldrich

Glutathione GSH/GSSG Assay Kit

Sufficient for 100 colorimetric tests

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Quality Level

100

application(s)

pharmaceutical

detection method

colorimetric

relevant disease(s)

Alzheimer′s disease; Parkinson′s disease; cancer; neurological disorders; aging/geriatric diseases

storage temp.

−20°C

Related Categories

General description

Glutathione, a tripeptide of glycine, glutamic acid, and cysteine, is one of the key antioxidants involved in protecting cells from damage by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG.

Features and Benefits

  • Linear detection range 0.01-3 μM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Suitability

The kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, tissue and cell extracts.

Principle

The Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman′s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at A412 nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Information

含少量动物源组分生物产品
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