MAK464
Lactate Dehydrogenase Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
LDH Assay Kit, Lactate Dehydrogenase Activity Assay Kit
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.84
Quality Level
application(s)
pharmaceutical
detection method
colorimetric
relevant disease(s)
cancer; hematological disorder
shipped in
ambient
storage temp.
−20°C
Related Categories
General description
Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage.
Application
The kit is suitable for lactate dehydrogenase activity determination in serum, plasma, and other sources. It is also suitable for the characterization and quality control of LDH production, as well as for the screening and evaluation of LDH modulators in drug discovery.
Biochem/physiol Actions
The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.
Features and Benefits
- Linear detection range 2-200 U/L
- Compatible with high-throughput handling systems.
- Simple procedure allows for reliable quantitation of LDH activity within 30 minutes.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
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