Skip to Content
Merck
CN

MAK464

Sigma-Aldrich

Lactate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

LDH Assay Kit, Lactate Dehydrogenase Activity Assay Kit

Sign Into View Organizational & Contract Pricing

Select a Size


About This Item

UNSPSC Code:
12352204
NACRES:
NA.84
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

application(s)

pharmaceutical

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder

shipped in

ambient

storage temp.

−20°C

General description

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage.

Application

The kit is suitable for lactate dehydrogenase activity determination in serum, plasma, and other sources. It is also suitable for the characterization and quality control of LDH production, as well as for the screening and evaluation of LDH modulators in drug discovery.

Biochem/physiol Actions

The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.

Features and Benefits

  • Linear detection range 2-200 U/L
  • Compatible with high-throughput handling systems.
  • Simple procedure allows for reliable quantitation of LDH activity within 30 minutes.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Regulatory Information

含少量动物源组分生物产品
常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service