Fatty acids are aliphatic monocarboxylic acids ubiquitously found in animal or vegetable fat, oil, and wax. Fatty acids play important roles in cellular synthesis, energy metabolism, and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome, and Reye Syndrome.

The kit is suitable for detecting free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, and cell cultures, and in food and agriculture products, as well as studying the effects of drugs on free fatty acid metabolism.

The Free Fatty Acid Assay Kit provides a simple, one-step and high-throughput assay for measuring Free Fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H²O². The resulting H²O² reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity (λEx = 530 nm/λEm = 585 nm) is directly proportional to the free fatty acid concentration in the sample.