MAK479

NADP/NADPH Assay Kit

Name: NADP/NADPH Assay Kit
Brand: SIGMA

sufficient for 100 colorimetric tests

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About This Item

NACRES:

NA.84

UNSPSC Code:

12161503

Key Documents

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Product Name

NADP/NADPH Assay Kit, sufficient for 100 colorimetric tests

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Related Categories

Biochemicals

Metabolism Assay Kits

Application

The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.

Biochem/physiol Actions

The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.

Features and Benefits

The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
Compatible with high-throughput handling systems.
Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.

General description

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.