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Merck
CN

MAK498

Alcohol Dehydrogenase (ADH) Assay Kit

sufficient for 100 colorimetric tests

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About This Item

NACRES:
NA.05
UNSPSC Code:
12161503
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detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

Quality Level

General description

Alcohol dehydrogenase (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.

Application

The kit is suitable for alcohol dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Biochem/physiol Actions

The non-radioactive, colorimetric Alcohol Dehydrogenase (ADH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Features and Benefits

  • Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
  • Compatible with high-throughput handling systems.

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Storage Class

3 - Flammable liquids

wgk

WGK 2

target_organs

Central nervous system

Regulatory Information

低风险生物材料
危险化学品
常规特殊物品
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