MAK524
Isocitrate Assay Kit
sufficient for 100 colorimetric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Quality Segment
application(s)
food and beverages
detection method
colorimetric
relevant disease(s)
cancer
shipped in
wet ice
storage temp.
−20°C
General description
Isocitrate (isocitric acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.
Application
The kit is suitable for isocitrate determination in food, beverages, and biological samples (e.g. cell lysate, tissue homogenate, serum, etc.).
Biochem/physiol Actions
The Isocitrate Assay Kit measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.
Features and Benefits
- Linear detection range: 20 to 5000 µM
- Compatible with high-throughput handling systems.
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Storage Class
12 - Non Combustible Liquids
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