Alkaline Phosphatase (ALP) Assay Kit

Name: Alkaline Phosphatase (ALP) Assay Kit
Brand: SIGMA

sufficient for 100 fluorometric tests

Synonym(s):

Fluorometric Alkaline Phosphatase Assay Kit

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About This Item

NACRES:

NA.84

UNSPSC Code:

12161503

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Properties

Quality Level

100

technique(s)

activity assay: suitable

application(s)

life science and biopharma

detection method

fluorometric

relevant disease(s)

cancer; neurological disorders; gastrointestinal diseases; genitourinary disorders

shipped in

wet ice

storage temp.

−20°C

Description

General description

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

Application

The kit is suitable for ALP activity in serum, plasma and other sources, characterization and quality control for ALP production, and for high-throughput screening for ALP modulators.

Biochem/physiol Actions

The Alkaline Phosphatase (ALP) Assay Kit utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into the highly fluorescent product 4methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.