MAK538
Ammonia Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Quality Segment
detection method
colorimetric, fluorometric
relevant disease(s)
gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
General description
Ammonia (NH3) or its ion form ammonium (NH4 +) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).
Application
The kit is suitable for ammonia determination in biological samples (e.g. serum, plasma, urine, saliva, cell culture, etc).
Biochem/physiol Actions
The Ammonia Assay Kit is designed to directly measure NH3 and NH4 +. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λEx = 360 nm/λEm = 450 nm is directly proportionate to the NH3 concentration in the sample.
Features and Benefits
- Linear detection range: 24 to 1000 µM
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Storage Class
10 - Combustible liquids
wgk
WGK 3
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